WEBTRIX™ PLATFORM TECHNOLOGY
Why extracellular microenvironment for cell culture
- Extracellular microenvironment, defined by biochemical cues and physical cues, is a deciding factor in a wide range of cellular processes.
- Cell proliferation, differentiation, migration, apoptosis or other specific functions is a coordinated response to the molecular interactions with these extracellular micro-environmental cues.
- in vitro cell culture environment that mimics the intricate and organized nanoscale meshwork of native ECM is desirable.
- An engineered native-like ECM is more likely to provide cells with rational cues for diagnostic and therapeutic studies
WebTrix™, a nanofiber offering in vivo like microenvironment
- in vitro cell culture environment that mimics the intricate and organized nanoscale meshwork of native ECM is desirable.
- An engineered native-like ECM is more likely to provide cells with rational cues for diagnostic and therapeutic studies
How to engineer in vivo like microenvironment
Nanofiber technology for physical cues control
MAPTrix™ ECM Library for biochemical cues control
MAPTrix™ ECM library is multiple-functional biomaterials that can be used as building blocks in a variety of life sciences applications such as design of artificial microenvironment including stem cell niche, tissue engineering scaffolds, and modification or coating for cell culture surface
PRODUCT LINES
Pre-coated Plate for Serum/Feeder-free Stem Cell Expansion
- Surface optimized for embryonic stem cell and/or induced pluripotent stem cell
- Applicable for murine & human stem cell
- Designed for serum-free, feeder-free culture
Pre-coated Plate for Stem Cell Study
- ECM motif binding to a variety of integrins highly expressed in embryonic stem cell or pluripotent stem cells
- Designed for stem cell study or its functions to elucidate and establish the relationship of cell surface receptors (integrins, syndecans, etc) to stem cell fate or behavior
WebTrix™ 3D Cell Culture plates
- Surface optimized for embryonic stem cell and/or induced pluripotent stem cell
- Ready to use; available without pre-coating step
- Designed for serum-free, feeder-free and Xeno-free culture
- Well defined 3D culture surface
- Surface optimized for embryonic stem cell and/or induced pluripotent stem cell
- Ready to use; available without pre-coating step
- Designed for serum-free, feeder-free and Xeno-free culture
- Well defined 3D culture surface
DATA
Mouse ES cell cultures on WebTrix™
- PDL: Poly-D-lysine
- Cell type: mouse embryonic stem cells (D3)
- Culture condition: Serum-free (15% Knockout serum replacement, DMEM), w/o LIF (essential factor for maintenance of mouse ESC)
WebTrix™, nanofiber based extracellular microenviornment, could support self-renewal and proliferation of mouse embryonic stem cell in feeder-free and serum-free media without LIF (leukemia inhibitory factor).
